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After maturation, human monocytes are released from bone marrow into the circulation as classical monocytes. Currently, strong evidence supports the concept that intermediate and non-classical monocytes emerge sequentially from the pool of classical monocytes (13). This is supported by transcriptome analysis showing that CD16+ monocytes have a more mature phenotype (14). In humans, 85% of the circulating monocyte pool are classical monocytes, whereas the remaining 15% consist of intermediate and non-classical monocytes (13). Classical monocytes have a circulating lifespan of approximately one day before they either migrate into tissues, die, or turn into intermediate and subsequently non-classical monocytes (6, 13). 2b1af7f3a8